p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas.
Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene.
The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression.
p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors.
Applications:Suitable for use in Flow Cytometry, Gel Supershift Assay, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 2ug/mg protein lystae, native and denatured, use Protein A.
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: 1-2ug/ml for 2 hrs at RT.
Optimal dilution determined by the researcher.
Positive Control: MDA-231, SKBR-3 cells or colon carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Specificity：Recognizes a 53kD human phosphoprotein, identified as p53 suppressor gene product. It reacts with mutant as well as wild form of p53. Species Crossreactivity: monkey and bovine. Reacts weakly with mouse and rat.
Clone # USB：DO-1
Isotype：IgG2a
Calc Applications Abbrev：E FC IF IHC IP WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Recombinant human wild type p53 protein expressed in E. coli. Cellular Localization: Nuclear Epitope: aa11-25 MW of antigen: 53kD
