85-7208-32　p53 (Tumor Suppressor Protein, Oncogene Protein) 500ul　P1001-32B

特徴

• p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas.
• Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene.
• The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression.
• p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors.
• Expression correlates with poor prognosis in breast cancer.
• In colonic cancer p53 expression seen in 47% of tumors and 9% of colonomas.
• p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
• Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis.
• p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
• DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to reduced interaction of p53 with its negative regulator, oncoprotein MDM2.
• MDM2 inhibits the accumulation of p53 by targeting it for ubiquitination and proteasomal degradation.
• p53 can apparently be phosphorylated by ATM, ATR and DNA-PK at Ser15 and Ser37; the phosphorylations impair the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.
• Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity.
• p53 is phosphorylated at Ser392 in vivo and by CAK in vitro.
• Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53.
• p53 is phosphorylated at Ser6 and Ser9 by ck1delta and ck1epsilon both in vitro and in vivo).
• Applications:Suitable for use in Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilution:Immunohistochemistry: Normal and neoplastic, formalin-fixed, paraffin embedded tissue sections.
• Optimal dilutions to be determined by the researcher.
• Positive Control: Colon
• Cellular Localization: Nuclear
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.