The epitope of anti-MyoD1 maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein and does not crossreact with myogenin, Myf5, or Myf6.
Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues.
MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas.
Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors.
Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.
Positive Control:SW80 cells
Applications:Suitable for use in Electron Microscopy, ELISA, Immunofluorescence and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 2ug/mg protein lysate.
Use Protein A.
Optimal dilutions to be determined by the researcher.
Cellular Localization:Nuclear.
Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide. See M9780-06X.
Specificity：Recognizes Human MyoD1. Does not crossreact with myogenin, Myf5 or Myf6. Species crossreactivity: mouse, rat and chicken.
Clone # USB：0.N.463 (5.2F)
Isotype：IgG2a
Calc Applications Abbrev：E IF IP
Calc Crossreactivity：Ch Hu Mo Rt
Immunogen：Recombinant protein corresponding to mouse MyoD1.
