BCNU) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA.
MGMT transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state.
This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate.
In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis.
Tumors with high levels of MGMT are likely be drug-resistant.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher.
Source:Ascites
Positive Control:Raji cells; Colon carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species Crossreactivity: Weakly crossreacts with mouse, rat and hamster.
Clone # USB：0.N.411
Isotype：IgG1
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221
