MMPs are frequently expressed in malignant neoplastic cells and are thought to play crucial roles in tumor invasion and metastasis.
Tissue inhibitors of matrix metalloproteinases (TIMPs) represent a family of ubiquitous proteins whose main biological function resides in their ability to control the activity of MMPS.
MMP secretion by microvascular endothelial cells is an essential initial step in the formation of new blood vessels (neo-angiogenesis).
Since angiogenesis does not occur in large blood vessels, it was investigated whether the secretion of MMPs and TIMPs differs between micro-and macro-vascular endothelial cells.
Cultured endothelial cells derived from microvascular vs macrovascular tissues exhibit different MMP and TIMP secretory profiles.
Applications:Suitable for use in ELISA, Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilutions:
Western Blot: 1ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:Human Endometrium cells.
Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells.
Placenta.
Bladder, breast, and ovarian carcinomas.
Cellular Localization:Cytoplasmic
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See M2421-10X.
Specificity：Recognizes human unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase—3 (MMP-3; also known as Stromelysin-1 or Transin).
Clone # USB：0.N.433 (SL-1 ID3)
Isotype：IgG1
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1).
