MMPs each have different substrate specificities within the ECM and are important in its degradation.
MMP-2 mainly degrades type IV collagen and denatured collagens.
MMP activity is modulated by tissue inhibitors of metalloproteinases (TIMP).
Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by TIMP have been implicated in the invasive potential of malignant tumors.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, and Western Blot.
Not suitable for Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoprecipitation (Native verified): 2ug/mg protein lysate.
Use Protein G.
Western Blot: 1-2ug/ml for 2 hrs at RT
Optimal dilution to be determined by the researcher.
Positive Control:Conditioned, serum-free medium from (TPA-treated) HFL-1 cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human MMP-2 pro form at 72kD and active form at ~66kD
