MELAN-A/MART-1 is a melanocyte differentiation marker.
MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines.
It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas.
MELAN-A/MART-1 is not expressed by non-melanoma malignancies.
It is recognized by autologous cytotoxic T lymphocytes.
Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
Seven other melanoma-associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1.
Applications:Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4.Also available with BSA and azide (Cat # M2410-12).
Specificity：Recognizes human MART-1. Species crossreactivity: equine.
Clone # USB：0.N.396 + 0.N.397
Isotype：IgG2b,k
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Eq Hu
Immunogen：Recombinant protein corresponding to human MART-1.
