85-7208-19　MART-1 (Melanoma Antigen Recognized by T-cells 1, Melan-A) (BSA & Azide Free) 100ug　M2410-11X

特徴

• MELAN-A/MART-1 is a melanocyte differentiation marker.
• MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines.
• It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas.
• MELAN-A/MART-1 is not expressed by non-melanoma malignancies.
• It is recognized by autologous cytotoxic T lymphocytes.
• Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
• Seven other melanoma-associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1.
• MART-1 (Melanoma Antigen Recognized by T-cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes.
• MART-1 is present in melanosomes and endoplasmic reticulum.
• Applications:Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilution:Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate
• Western Blot: 1ug/ml for 2 hours at RT.
• Immunohistochemistry (Frozen & formalin/paraffin): 1-2ug/ml for 30 min.
• at RT.
• Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
• Optimal dilutions to be determined by the researcher.
• Positive Control:CaC1 melanoma cells and melanomas.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.