MART-1 (Melanoma Antigen Recognized by T-cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes.
MART-1 is present in melanosomes and endoplasmic reticulum.
Applications:Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate
Western Blot: 1ug/ml for 2 hours at RT.
Immunohistochemistry (Frozen & formalin/paraffin): 1-2ug/ml for 30 min.
at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:CaC1 melanoma cells and melanomas.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.Also available without BSA and azide. See M2410-11X.
Specificity：Recognizes human MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Does not crossreact with MAGE-1 or tyrosinase protein. Species Crossreactivity: Canine
Clone # USB：0.N.399
Isotype：IgG1
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Ca Hu
Immunogen：Recombinant protein corresponding to human MART-1. Cellular Localization: Cytoplasmic.
