85-7208-17　MART-1 (Melanoma Antigen Recognized by T-cells 1, Melan-A) 500ul　M2410

特徴

• MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes.
• Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1.
• Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
• Applications:Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunoprecipitation (Denatured verified): 2ug/mg protein lysate.
• Use Protein A.
• Western Blot: 0.5-1ug/ml for 2 hours at RT.
• Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT.
• Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
• M2410-12 is preferred for this application.
• Optimal dilutions to be determined by the researcher.
• Positive Control:CaC1 melanoma cells and melanomas
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.