The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:IMR-5 or T98G human glioblastoma cells or brain
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2350-01B.
Specificity：Recognizes the phosphorylated form of bovine MAP2a,b,c and shows no crossreactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Reacts with dendrites and cell bodies of neurons. Species Crossreactivity: human, rat, Xenopus and quail.
