85-7208-11　Lck, p56 (Lymphocyte-specific Tyrosine Kinase) (BSA & Azide Free) 100ug　L1565-01X

特徴

• p56lck is a membrane-associated non-receptor protein tyrosine kinase from the Src family of kinases.
• The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase.
• p56lck plays a critical role in T cell development and activation.
• p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas.
• Applications:Suitable for use in ELISA, Immunoprecipitation, Kinase Assay, and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Immunoprecipitation: Use Protein A.
• 2ug antibody/mg protein lysate
• Western Blot: 1-2ug/ml for 2 hours at room temperature.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Jurkat cells
• Cellular Localization:Cell membrane
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.