Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains.
They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG).
All interact specifically with each other to form a continuous and regular BM.
Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Immunohistochemistry (Acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 minutes at RT.
Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive Control:LS174T cells, normal colon or colon carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.Also available without BSA and azide. See L1227-10X.
Specificity：Recognizes the mouse laminin B2/g1 chain at ~210kD. Species Crossreactivity: human
