85-7208-07　Keratin, pan multiple clones 500ul　K0199-91

特徴

• Keratins are resolved with two dimensional gel electrophoresis into acidic pH, <5.7, and basic, pH >6.0 subfamilies.
• Members of the acidic and basic subfamilies are found together in pairs.
• The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state.
• Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
• Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Frozen & formalin/paraffin): 1-2ug/ml.
• For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT, or Pronase E at 1mg/ml PBS for 5 minutes at 37°C.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Skin
• Cellular Localization:Cytoplasmic
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.