Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies.
The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kD.
The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD.
Members of acidic and basic subfamilies are found together in pairs.
The composition of keratin pairs varies with cell type, differentiation status and environment.
Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Applications:Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT.
For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 minuts at 37°C or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive Control:Skin.
Lung carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Purified
Purity：Purified from ascites
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA. See K0199-75X
Specificity：Recognizes the 65-67, 64, 59, 58, 56 and 52kD human keratins of the basic subfamily. Species Crossreactivity: monkey, bovine, rabbit, mouse, rat and chicken
