85-7208-03　Keratin 20 500ul　K0199-25

特徴

• CK20 is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells.
• CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system.
• CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas.
• Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-2ug/ml for 2hrs at RT
• Immunohistochemistry (formalin/ paraffin): 1:50-1:100 for 30 min at RT.
• Requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
• Optimal dilutions to be determined by the researcher.
• Positive Control:HT29 cells.
• Colon carcinoma.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.