Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments.
it is expressed in various different human tissues except in liver.
Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT.
Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
(Protease XXIV damages the epitope recognized by this antibody).
Western Blot: 2-4ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:LS174T and HT29 cells, skin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes the rod domain of human keratin 19 (40kD). Reacts with the MCF-7 cells, which are known to contain keratin 19. It shows no reaction with the cells lacking keratin 19 such as A431 and HeLa. Weak reactivity with rat, mouse and guinea pig.
Clone # USB：0.N.365
Isotype：IgG2a
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Human breast cancer MCF-7 cells. Epitope: aa312-335 Cellular Localization: Cytoplasmic. MW of Antigen: 40kD
