85-7207-99　［取扱停止］Keratin 17 500ul　K0199-20

特徴

• The type I keratin 17 (K17) shows a peculiar localization in human epithelial appendages including hair follicles, which undergo a growth cycle throughout adult life.
• Additionally K17 is induced, along with K6 and K16, early after acute injury to human skin.
• Predominant expression of K17 and the frequent expression of K8 and K19, with little K6/K16 and K1/K10 expression are the characteristic features of basal cell carcinomas (BCC).
• This expresssion indicates BCC is differentiated towards undifferentiated follicular epithelia, most probably hair bulge cells.
• Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilution:Immunohistochemistry (frozen & formalin/paraffin): 1:40-1:80 for 30 min at RT.
• Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Squamous cell carcinoma or skin
• Cellular Localization:Cytoplasmic
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.