Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins).
Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas.
Only 10% of the invasive breast carcinomas show diffuse or focal positivity.
Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67.
This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry (formalin/paraffin).
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1-2ug/ml for 30 min.
at RT.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positve Control:Squamous cell carcinoma
Cellular Localization:Cytoplasmic
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
Specificity：Recognizes human keratin 16 at 48kD. Does not react with rat.
Clone # USB：0.N.361
Isotype：IgG1
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues from the C-terminus of human cytokeratin 16
