Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis.
In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer.
Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Applications: Immunofluorescence, Western Blotting, Immunohistochemistry.
Recommended Dilution:Western Blotting: 1ug/ml
Immunohistochemistry: 2-4ug/ml.
Frozen & formalin/paraffin.
No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available with BSA and azide. See K0199-12.
Specificity：Recognizes keratin 10 at 56.5kD. Species crossreactivity: Human, rat, canine and feline.
Clone # USB：0.N.354 (DE-K10)
Isotype：IgG1
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Ca Fe Hu Rt
Immunogen：Cytoskeletal preparation extracted from human ectocervical epithelium.
