Keratin 10 (Cytokeratin 10, CK 10) is expressed in all suprabasal layers of the epidermis.
In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer.
Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-2ug/ml for 2 hours at RT
Immunohistochemistry (formalin/paraffin): 0.5-1ug/ml for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:A431 and skin cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. See K0199-12X.
Specificity：Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.
Clone # USB：0.N.354 (DE-K10)
Isotype：IgG1
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Ca Fe Hu Rt
Immunogen：Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.
