85-7207-92 Keratin 8 500ul K0199-10
特徴
- Applications:Suitable for use in Immunohistochemistry (formalin/paraffin) and Immunoprecipitation.
- Other applications have not been tested.
- Recommended Dilution:Immunoprecipitation: 2ug antibody/mg protein lysate.
- Use Protein G.
- Immunohistochemistry (formalin-fixed, paraffin embedded tissues): 0.5-1ug/ml for 30 mins.
- at RT.
- Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：500ul
- Host：mouse
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by Protein G affinity chromatography.
- Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
- Specificity：Recognizes human keratin 8 at 52.5kD. The epitope maps between residues 343-357 of the protein. Does not react with Rat.
- Clone # USB：0.N.351
- Isotype：IgG1,k
- Calc Applications Abbrev：IHC IP
- Calc Crossreactivity：Hu
- Immunogen：Keratin preparation from a human carcinoma.
- Shelf Life：1year
- EU Commodity Code：30021010
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