Reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
The most common feature of these malignancies is the restricted expression of a single light chain class.
Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (frozen and formalin/paraffin): 1:400-1:800 for 30 min at RT.
Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. Also available with BSA and azide. See K0080.
Specificity：Highly specific to the kappa light chain of immunoglobulin.Species Crossreactivity: Human. Does not react with Rat.
Clone # USB：0.N.343
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：B lymphoma cells. Cellular Localization: Cytoplasmic.MW of antigen: 25kD
