85-7207-88　［取扱停止］Hypoxia Inducible Factor 1a (HIF-1a) 100ul　H9807

特徴

• Hypoxia-inducible factor-1 (HIF-1).
• HIF-1 is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors.
• HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation.
• HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1.
• The C-terminal of HIF-1 alpha binds to p300.
• p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: paraffin
• Optimal dilutions to be determined by the researcher.
• Positive Control:Mammalian cells cultured under reduced O2 tension.
• Cellular Localization:Nuclear
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.