85-7207-86　［取扱停止］Histone H1 500ul　H5110-01A

特徴

• In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter.
• They consist of two major components in equal amounts, DNA and basic proteins called histones.
• The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes.
• They are characterized by relatively high levels of lysine and arginine.
• Although histones are classified into a limited number of types of fractions (see below) with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues.
• Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development.
• They are believed to play a role in gene activity and cellular metabolism.
• Histones are believed to be regularly arranged in the deep groove of the DNA helix.
• The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible.
• This allows for the supercoiling of the chromatin fibers.
• Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
• Not suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Immunohistochemistry (formalin/paraffin): 2-4ug/ml.
• No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues
• Optimal dilutions to be determined by the researcher.
• Positive Control:Tonsil or lymph node
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.