Heat shock proteins, HSP’s or stress response proteins SRP’s, are synthesized in a variety of environmental and patholphysiological stressful conditions.
Many HSP’s are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion.
HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins.
Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.
Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Native & denatured): 2ug antibody/mg protein lysate using Protein A.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:BT474 or HeLa cells, breast carcinoma.
Cellular Localization:Nuclear and cyotplasmic
Molecular Weight of Antigen:Inducible: 72kD
Constitutive: 73Kd
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4.
Specificity：Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species crossreactivity: Human, monkey and bovine.
