A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of heat shock proteins (hsp’s) or stress response proteins (srp’s).
hsp60 is a potential antigen in a number of autoimmune diseases including human arthritis.
H1830-75X is useful in distinguishing hsp60 from mammals and bacteria.
Applications:Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:BT474 cells, MAD109 cells and breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See H1830-75.
Specificity：Recognizes human Heat Shock Protein 60 at ~60kD. Species Crossreactivity: mouse, rat, hamster, mammal and chicken. Does not react with bacteria, helminths or spinach.
