The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al.
2008).
~75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy (Bhargava et al.
2012).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Frozen): 1:40-1:60, the use of Zamboni's fixative is recommended
Immunohistochemistry (Paraffin): 1:40-1:80, requires antigen retrieval using heat treatment prior to staining of paraffin sections.
It is recommended to use sodium citrate buffer pH 6.0 for this purpose.
Western Blot: 1:50-1:100 for 2 hrs.
at RT
Immunoprecipitation (denatured verified): 10ul/mg protein lysate.
Use Protein G.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human Estrogen Receptor at 67kD. Species Crossreactivity: rat
Clone # USB：6F11
Isotype：IgG1
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to full length human estrogen receptor.
