85-7207-69　Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3) 500ul　C8454-20

特徴

• Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2.
• Cyclins D1, D2, and D3 form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
• Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunoprecipitation (Native): 2ug/mg protein lysate.
• Use Protein G
• Western Blot: 1-2ug/ml for 2 hrs at RT.
• Immunohistochemistry (formalin/paraffin): 1:25 for 60 minutes at RT.
• Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
• Optimal dilutions to be determined by the researcher.
• Positive Control:LS174T cells, breast carcinoma or tonsil.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.