Optimal dilutions to be determined by the researcher.
Positive Control:Normal skin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ul
Host:mouse
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:Recognizes human intact (250kD) and collagenase digested (150kD) tissue Type VII collagen. Does not crossreact with types I, II, III, IV, V collagens, lamins or fibronectin. Epitope: Located on non-helical (non-collagenous) C-terminal region of collagen VII and is resistant to collagenase, trypsin, pronase, neuraminidase, chondroitinase, and hyaluronidase. It is, however, lost by digestion of collagen VII with pepsin. Species Crossreactivity: marmoset, monkey, bovine, sheep, goat, porcine and guinea pig.
Clone # USB:0.N.229
Isotype:IgG1,k
Calc Applications Abbrev:E IF IHC
Calc Crossreactivity:Bo Gt Gp Hu Mk Po Sh
Immunogen:Insoluble fractions of human neonatal foreskin epidermal cells. Cellular Localization: Epidermal basement membrane. MW of Antigen: 250kD (Intact); 150kD (Digested)