Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Not suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive Control:Cartilage
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：mouse
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19.
Specificity：Recognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. Species Crossreactivity: human and mouse.
Clone # USB：0.N.225
Isotype：IgG2a,k
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Ch Hu Mo
Immunogen：Purified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix.
