Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT.
Note: Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive Control:Cartilage in lung or fetus
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19A.
Specificity：Recognizes chicken Type II Collagen. Species Crossreactivity: human, mouse, salamander. Reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. Immunoblotting of CNBr peptides of collagen II shows that this reacts with CB 11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In Western Blot (but not ELISA), this recognizes a1(II) as well as a3(XI) chain both of which have identical primary structure.
Clone # USB：0.N.226
Isotype：IgG1
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Ch Hu Mo
Immunogen：Purified type II collagen obtained from the adult chicken sternum by limited pepsinization. Epitope: Triple helix of type II collagen. Cellular Localization: Cartilage matrix
