85-7207-58　［取扱停止］Chorionic Gonadotropin, Human, beta (hCGb) multiple clones (+2) (BSA & Azide Free) 100ug　C5069-63X

特徴

• Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts.
• It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy.
• HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits.
• The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
• A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.
• Applications:Immunocytochemistry, Radioimmunoassay, Immunohistochemistry (frozen & formalin-fixed).
• Not recommended for Western Blotting.
• Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min.
• Recommended Dilution:Immunohistochemistry: 1-2ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.