A family of proteins designated as cdks are critical regulators of cell cycle progression.
The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation Native only; coprecipitates cyclin D’s: 2ug/mg protein lysate.
Use protein G
Western Blot: 1-2ug/ml for 2 hours at RT.
Optiml dilutions to be determined by the researcher.
Positive Control:MAD109, NIH3T3, LS174T, and MCF-7 cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes p34 (34kD). Does not crossreact with other cdks. Species Crossreactivity: Human, mouse, rat and porcine.
