CD163 antigen is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4.
CD163 is restricted in its expression to the monocytic/macrophage lineage.
It is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan’s cells.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 60 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:Placenta
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human CD163. May be useful in the localization of monocytes and most macrophage types as well as aiding further study into the function and expression of the antigen itself.
Clone # USB：0.N.188
Isotype：IgG1
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein encoding domains 1 to 4 of the N-terminal region of human CD163. Cellular Localization: Cell membrane
