Thrombomodulin (also known as Fetomodulin) contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein.
Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance, thus reducing clot formation on the surface of the endothelial cells.
Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 60 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:Mesothelioma, Lung and Placenta
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human Thromomodulin. Species Crossreactivity: rat
Clone # USB：0.N.186
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to the six repeated EGF domains of human thrombomodulin. Cellular Localization: Cell membrane
