CD137 (also known as 4-1BB) is a glycoprotein (aa256) of 30kD.
It exists as both a monomer and a 55kD dimer on the surface of activated T cells.
4-1BB is an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes.
4-1BB is structurally related to the members of NGFR/TNFR superfamily which are characterized by the presence of three-six patterns of a cysteine-rich motif in their extracellular domains.
Other members of this family include low affinity NGFR, two receptors for TNF (TNFR-I & TNFR-II), CD30, CD40, OX40, Fas and CD27.
These molecules are involved in cell growth, survival and death processes.
The cytoplasmic domain of 4-1BB include two runs of acidic amino acids, a potential p56lck binding site, five consecutive glycines at the C-terminus, and four potential phosphorylation sites: one tyrosine, two threonine, and one serine.
Applications:Suitable for use in Flow Cytometry and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Control:Spleen, thymus, or tonsil.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2548-50.
Specificity：Recognizes human CD137 at 30kD (monomer) and 55kD (dimer).
Clone # USB：0.N.185
Isotype：IgG1,k
Calc Applications Abbrev：FC IF
Calc Crossreactivity：Hu
Immunogen：Ectodomain of human 4-1BB recombinant protein (30kD). Cellular Localization: Cell membrane. Epitope: Ectodomain.
