CD117 or c-kit is a transmembrane receptor tyrosine kinase.
It binds to stem cell factor (SCF).
It is expressed in many tissues and cells.
C-kit/SCf is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes and hematopoietic precursor cells
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Frozen or fixed in methacarn or Stieves and embedded in paraffin
Optimal dilutions to be determined by the researcher.
Positive Control:Skin, GI Stromal tumor (GIST)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human CD117/c-kit. Does not interfere with the binding of SCF to c-kit.
Clone # USB：0.N.183
Isotype：IgG2a
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant human extracellular c-kit fragment. Epitope:Extracellular second N-terminus Ig-like domain of c-kit. Cellular Localization: Cell membrane/cytoplasmic
