CD99 or MIC2 gene product, or E2 antigen is expressed in the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary.
The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis and some endothelial cells.
Mature granulocytes express very little or no CD99.
MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT.
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Pancreas or Ewing’s sarcoma
Cellular Localization:Cell membrane
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Specificity：Recognizes human CD99/MIC2/E2 at 27-32kD. Species Crossreactivity: Rat.
Clone # USB：0.N.171 (HO36-1.1)
Isotype：IgM
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Rt
Immunogen：Purified E-rosette forming cells from human peripheral blood lymphocytes.
