Complement receptor 1 (CR1, CD35) is a cell membrane-bound, monomeric glycoprotein.
It is found on numerous cell types of primates including erythrocytes, lymphocytes and phagocytes.
The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules.
Applications:Suitable for Flow Cytometry, Immunofluorescence, Immunohistochemistry.
Not suitable for use in Western Blot and Immunoprecipitation.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Epitope: Distal from C3b- binding site
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide at ~1mg/ml. Refer to C2387-10X.
Specificity：Recognizes human complement receptor 1 (CR1)/CD35 (210-220kD). Specific for a site that is distal from the C3b-binding site. Does not block CR1 activity. Does not crossreact with CR2. Species Crosseactivity: Rhesus Monkey and Baboon.
Clone # USB：0.N.104 (E11)
Isotype：IgG1
Calc Applications Abbrev：FC IF IHC
Calc Crossreactivity：Hu Mk
Immunogen：Intact human monocytes. Epitope: Distal from C3b- binding site
