CD25, otherwise known as the Tac antigen or p55, is the alpha chain of the Interleukin-2 (IL-2) receptor.
The IL-2 receptor is expressed on activated cells including T cells, B cells and monocytes.
It is also present on a subset of thymocytes, HTLV-1 transformed T and B cells, EBV transformed B cells, myeloid precursors and oligodendrocytes.
IL-2 induces the expression of the CD25 subunit on NK cells.
The IL-2 receptor consists of at least three distinct membrane components: the alpha chain (IL-2R-alpa, Tac, CD25), the beta chain (IL-2R-beta, p75, CD122), and the gamma chain (IL-2R-gamma).
CD25 is undetectable on resting T cells but is induced upon T-cell activation.
Applications:Suitable for use in Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:Tonsil or lymph node
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2276-10X.
Specificity：Recognizes human interleukin 2 receptor alpha (IL-2Ra) (also known as T-cell growth factor receptor or CD25) at 55kD.
Clone # USB：0.N.93 (IL2R.1)
Isotype：IgG1,k
Calc Applications Abbrev：FC IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human interleukin 2 receptor.Cellular Localization: Cell membrane
