CD21 is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes.
In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells.
CD21 expression is also gradually lost after stimulation of B cells in vitro.
CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.
CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes., followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes human CD21 at ~140kD.
Clone # USB：0.N.89
Isotype：IgG2a
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein encoding the external domain of human CD21. Cellular Localization: Cell membrane.
