CD8 molecule consists of two chains, termed a and B chain, which are expressed as a disulphide-a b heterdimer or as an alpha a homodimer on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.
The majority of CD8+ Tcells expresses CD8 as abheterodimer.
CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex.
The HIV-2 envelope glycoprotein binds CD8 a chain (but not B chain).
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 20 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Hybridoma:p3-NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.
Positive Control:Lymph node or tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human CD8 at ~32kD. Does not react with rat.
Clone # USB：0.N.66 (C8/144B)
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from the C-terminal cytoplasmic domain of the alpha chain of human CD8. Cellular Localization: Cell membrane
