The CD5, a transmembrane protein, is found on 95% of thymocytes and 72% of peripheral blood lymphocytes.
In lymph nodes, the main reactivity is observed in T cell areas.
CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells.
Occasionally, CD5 antigen is also expressed on a subset of B cells.
Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.
Applications:Suitable for use in Immunohistochemistry (formalin/paraffin only).
Not recommended for Western Blotting.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:40 for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Tonsil
Hybridoma:p3-NS1.Ag4-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a liquid, 0.09% sodium azide
Specificity：Recognizes human CD5 at 67kD. Does not react with Rat.
Clone # USB：4C7
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to the external domain of the CD5 molecule.
