CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells.
Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X).
p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation.
CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy.
Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells.
The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation.
Applications:Suitable for use in Immunohistochemistry.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher
Positive Control:Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes human CD2 at 45-58kD.
Clone # USB：0.N.46
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant fragment encoding the external domain of the human CD2 protein. Epitope: Extracellular domain. Cellular Localization: Cell membrane
