Calponin, a calmodulin, binds tropomyosin and F-actin and is thought to be involved in the regulation of smooth muscle contraction.
The expression of calponin is restricted to smooth muscle cells and is a marker of the differentiated contractile phenotype of developing smooth muscle.
Two isoforms of calponin exist whose molecular weights are 34kD and 29kD.
Expression of the 29kD form is primarily restricted to muscle of the urogenital tract.
The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Myoepithelial cells in breast ducts.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes the 34kD isoform of human Calponin. Species Crossreactivity: Rat.
Clone # USB：0.N.39 (CALP)
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Crude human uterus extract. Cellular Localization: Cytoplasmic. MW. of Antigen: 34kD.
