c-myc is involved in the control of cell proliferation and differentiation.
Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (k light chain); or 22 (l light chain) is described in Burkitt’s lymphoma and other B-cell lympho-proliferative conditions.
An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Electron Microscopy and Affinity Purification.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (frozen & formalin/paraffin): 2-4ug/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Western Blot: 1-2ug/ml for 2hours at RT on fusion proteins labeled with EQKLISEEDL tag.
Affinity Purification and Immunoprecipitation: Use on fusion proteins labeled with EQKLISEEDL tag.
Optimal dilutions to be determined by the researcher.
Positive Control:Raji cells.
Breast carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C0035-10X
Specificity：Recognizes human c-myc at 64-67kD (EQKLISEEDL). Does not cross-react with mouse, rat or chicken.
Clone # USB：0.N.222 (9E10.3)
Isotype：IgG1,k
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from the C-terminus of human c-myc. Epitope: 410-419. Cellular Localization: Nuclear.
