c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR.
C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors.
Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer.
Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Applications:Suitable for use in Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation (Denatured): 1:25 protein lysate using Protein G.
Immunohistochemistry: 1:1000-1:2000 for 20 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:SKBR-3 cells, MAD109 cells, breast carcinomas
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C0015-20X.
Specificity：Recognizes human c-erbB-2 (~185kD) in both the wild and mutant (oncogenic) form. Preferentially recognizes the unphosphorylated form.
Clone # USB：0.N.211
Isotype：IgG1
Calc Applications Abbrev：IHC IP
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region of the C-terminus of human c-erbB-2 protein. Cellular Localization: Cell membrane. Species Sequence Homology: rat, 100%
