Bcl-X has 2 isoforms, Bcl-Xl (long) a 241 amino acid protein and Bcl-Xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family.
Applications:Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipiation and Immunohistochemistry.
Not suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Use Protein A): 2ug/mg protein lysate
Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30min at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:Hodgkin’s lymphoma
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.Also available with BSA and azide. See B0807-14.
Specificity：Recognizes Bcl-X at 27kD. Does not react with Bcl-2a or Bax protein. Species Crossreactivity: Human, mouse, rat and porcine.
Clone # USB：2H12
Isotype：IgG2a
Calc Applications Abbrev：E FC IF IHC IP
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to residues 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. Cellular Localization: Cytoplasmic and cell/nuclear membrane.
