Bcl 6 is mainly expressed in normal germinal center B cells and related lymphomas.
Bcl 6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas and Bcl 6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas.
Bcl 6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas and in nodular, lymphocyte predominant Hodgkin’s disease
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/Paraffin): 1:10 for 60 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 minutes, followed by cooling at RT for 20 minutes.
Requires use of a high sensitivity detection system.
Optimal dilutions to be determined by the researcher.
Positive Control: Tonsil
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes human Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein)(95kD). Species Crossreactivity: Bovine, rabbit, rat, sheep and porcine.
Clone # USB：0.N.26 (BL6.02)
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Bo Hu Po Rb Rt Sh
Immunogen：Recombinant protein corresponding to aa3-484 of the human Bcl-6 protein. Cellular Localization: Nuclear. Epitope: aa3-484.
